Human carbonic anhydrase isozyme II has been used to assess its gene fusion abilities for efficient expression and recovery of recombinant proteins. Human carbonic anhydrase isozyme II has also been used to investigate a new process for the synthesis of difluoromethanesulfonamides 1. Proteins. 1988;4(4):274-82. Refined structure of human carbonic anhydrase II at 2.0 A resolution. Eriksson AE(1), Jones TA, Liljas A. Author information: (1)Department of Molecular Biology, Biomedical Center, Uppsala, Sweden. The structure of human erythrocytic carbonic anhydrase II has been refined by constrained and restrained structure-factor least-squares refinement at 2.0 A resolution 5dsi, 5dsj: Kim JK, Lomelino CL, Avvaru BS, Mahon BP, McKenna R, Park SY & Kim CU (2018) Active-size solvent replenishment observed during human carbonic anhydrase II catalysis. IUCrJ 5, 93-102. BC Tripp, K Smith & JG Ferry (2001) Carbonic anhydrase: New insights for an ancient enzyme. Journal of Biological Chemistry 276, 48615-48618
, including anti-carbonic anhydrase II (CAII) autoantibodies, have been found in patients with cancer-associated retinopathy and autoimmune retinopathy without diagnosed cancer Carbonic anhydrase 2 (EC: 220.127.116.11) Alternative name(s): Carbonate dehydratase II. Carbonic anhydrase C. Short name: CAC. Carbonic anhydrase II. Short name: CA-II Gene names i: Name:CA2. Organism i: Homo sapiens (Human) Taxonomic identifier i: 9606 : Taxonomic. Carbonic anhydrase II (CA II) is one of 14 isozymes of carbonic anhydrases, zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate. Mutations in CA II in humans lead to osteopetrosis with renal tubular acidosis and cerebral calcifications, a disorder often associated with mental retardation
Carbonic anhydrase (CA) is a metalloenzyme which is widely distributed in the living world, and it is essential for the regulation of acid-base balance. Anti-CA antibodies have been reported in many disorders, such as systemic lupus erythematosus, Sjögren's syndrome, rheumatoid arthritis, endometriosis, idiopathic chronic pancreatitis, type 1 diabetes and Graves' disease Purpose: Carbonic anhydrases (CAs) are key enzymes that regulate acid-base homeostasis in both normal and pathological conditions. Recent studies have shown that they are functionally involved in the growth and invasion of cancer cells. However, there are only a few publications on CAs in hematological malignancies. Experimental Design: Here we investigated the presence of CA isozymes in six. Electrophoretic variants of carbonic anhydrase II (formerly called carbonic anhydrase C and later carbonic anhydrase B) were described by Moore et al. (1971) in American blacks. Several variants of carbonic anhydrase II have been characterized in humans. Tashian et al. (1968) found CA II variants in monkeys Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube Carbonic anhydrase, a zinc metalloenzyme, catalyzes the reversible hydration of carbon dioxide to bicarbonate. It is involved in processes connected with acid-base homeostasis, respiration, and photosynthesis. More than 100 distinct human carbonic anhydrase II (HCAII) 3D structures have been generated in last 3 decades [Liljas A, et al..
Donate here: http://www.aklectures.com/donate.php Website video link: http://www.aklectures.com/lecture/carbonic-anhydrase Facebook link: https://www.faceboo.. The structure of human erythrocytic carbonic anhydrase II has been refined by constrained and restrained structure-factor least-squares refinement at 2.0 A resolution. The conventional crystallographic R value is 17.3%. Of 167 solvent molecules associated with the protein, four are buried and stabilize secondary structure elements Carbonic Anhydrase II is essential for bone resorption and osteoclast differentiation by similarity. It can hydrate cyanamide to urea and is involved in the regulation of fluid secretion into the anterior chamber of the eye. Anti-Carbonic Anhydrase II Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal. Invitrogen Anti-Carbonic Anhydrase II Monoclonal (11A1), Catalog # MA5-34925. Tested in Western Blot (WB), Immunocytochemistry (ICC) and Immunohistochemistry (Paraffin) (IHC (P)) applications. This antibody reacts with Human samples. Supplied as 100 µL purified antibody (2 mg/mL)
Recombinant Human Carbonic anhydrase II Protein (Met1-Lys260) 10478-H08E with a fusion His Tag, is expressed in E. coli. With high purity, high biological activity, high stability, and other superior features, you can use this Human Carbonic anhydrase II protein for relevant bioassay and related research Carbonic anhydrase 2, Carbonate dehydratase 2, Carbonic Anhydrase II, CA-II, Carbonic anhydrase C, CAC, CA2, CAII, Car2. Introduction The enzyme Carbonic anhydrase II having an accession number of NP_414668 is also called carbonate dehydratase which is part of the enzyme family that catalyses rapid inter-conversion of carbon dioxide & water to bicarbonate, carbonic acid and protons (CO2 + H2O Carbonic anhydrases constitute a group of enzymes that catalyse reversible hydration of carbon dioxide leading to the formation of bicarbonate and proton. The platelet carbonic anhydrase II (CAII) was described for the first time in the '80s of the last century. Nevertheless, its direct role in platelet physiology and pathology still remains poorly understood 33 antibodies to Carbonic Anhydrase II and validated for use in 9 applications (Immunohistochemistry, Dot blot, Western Blot, Immunoprecipitation, Flow Cytometry, Immunocytochemistry, Immunodiffusion, Immunofluorescence, ELISA Background: Carbonic Anhydrase II/CA2 Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO 2 + H 2 O = HCO 3 - + H + , which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1)
Carbonic anhydrase, enzyme found in red blood cells, gastric mucosa, pancreatic cells, and renal tubules that catalyzes the interconversion of carbon dioxide (CO2) and carbonic acid (H2CO3). Carbonic anhydrase plays an important role in respiration by influencing CO2 transport in the blood. Th Carbonic anhydrase (CA) catalyzes the reversible hydration and dehydration reactions of CO 2 /H 2 CO 3. CAs are widespread in nature, being found in animals, plants, and certain bacteria. Sixteen isozymes have been identified and characterized in mammals CARBONIC anhydrase, which catalyses the reversible reaction CO 2 + H 2 ⇄ H 2 CO 3, is an intracellular enzyme found in high concentration within red blood corpuscles 1. The activity of this enzyme..
Carbonic anhydrase IX (CA IX) is a membranously located metalloenzyme involved in pH homeostasis with the influence on regulation of cell proliferation, oncogenesis, and tumor progression. Blood Oxygenation Level-dependent Magnetic Resonance Imaging of Breast Cancer: Correlation with Carbonic Anhydrase IX and Vascular Endothelial Growth Facto There are no reviews for Carbonic Anhydrase II/CA2 Recombinant Protein (NBC1-28002). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount. Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen; Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Ye Background and purpose Although it has been reported that bovine carbonic anhydrase CAII is capable of generating NO from nitrite, the function and mechanism of CAII in nitrite‐dependent NO formation and vascular responses remain controversial
CAII - Carbonic Anhydrase Isoenzyme II. Looking for abbreviations of CAII? It is Carbonic Anhydrase Isoenzyme II. Carbonic Anhydrase Isoenzyme II listed as CAI Human carbonic anhydrase II, solid surfaces, adsorption, applications Sammanfattning The adsorption of proteins to solid surfaces has been extensively investigated during the past 20-30 years. The knowledge can be applied in biotechnological applications in for example immunoassays and biosensors For the biopharma industry investment, business development and competitive intelligence professionals who require information to support financing, partnering and licensing activities, BCIQ provides accurate information and context to support profitable and strategic decision making. Unlike other intelligence solutions, BCIQ exclusively supports the unique needs of the biopharma industry and. Venta PJ, Welty RJ, Johnson TM, Sly WS, Tashian RE: Carbonic anhydrase II deficiency syndrome in a Belgian family is caused by a point mutation at an invariant histidine residue (107 His----Tyr): complete structure of the normal human CA II gene. Am J Hum Genet. 1991 Nov;49(5):1082-90. [PubMed:1928091
Structure of Carbonic Anhydrase II The -class of CAs is comprised of monomers and has a molecular weight of 30000 Da. CA II is a globular protein that measures approximately 5 x 4 x 4 nm (Lindskog, 1997) The central structural motif of hCA II is characterized by a 10 -stranded ( A J) twisted -sheet,. Definition of carbonic anhydrase ii in the Definitions.net dictionary. Meaning of carbonic anhydrase ii. What does carbonic anhydrase ii mean? Information and translations of carbonic anhydrase ii in the most comprehensive dictionary definitions resource on the web Human carbonic anhydrase II and rat liver arginase serve as well-studied paradigms of zinc and manganese metalloenzymes, respectively . In 1983, we reported a deficiency of carbonic anhydrase II, one of the isozymes of carbonic anhydrase, in three sisters with this disorder Yeger, Carbonic anhydrase II mediates malignant behavior of pulmonary neuroendocrine tumors, American Journal of Respiratory Cell and Molecular Biology, vol. Biophysical, Biochemical, and Cell Based Approaches Used to Decipher the Role of Carbonic Anhydrases in Cancer and to Evaluate the Potency of Targeted Inhibitors
Carbonic anhydrase (CA) are enzymes which catalyze the interconversion of CO2 and water to bicarbonate and proton. The active site contains Zn atom. CA enzymes are divided into several classes: α-carbonic anhydrase are found in mammals. β-carbonic anhydrase are found in prokaryotes A significant upregulation of carbonic anhydrase II at the mRNA and protein levels was observed upon treatment with polyinosinic-polycytidylic acid, a toll-like receptor 3 agonist. Furthermore, in agreement with the increased expression of carbonic anhydrase II in atopic dermatitis skin, carbonic anhydrase II was upregulated by the Th2 cytokines interleukins -4 and -13 A deficiency of carbonic anhydrase II can result in osteopetrosis and metabolic acidosis. The inhibition of carbonic anhydrase IV and possibly carbonic anhydrase II by sulfonamides is a current therapy in the treatment of glaucoma. Synonym(s): carbonate dehydratase, carbonate hydro-lyase Figure 2.2 - pH dependence of k cat and K m values for CO 2 hydration catalyzed by carbonic anhydrase I, II, and III isoenzymes. 33 ,36 In vitro , carbonic anhydrase is quite versatile, catalyzing several reactions that involve both OH - and H + , such as the hydrolysis of esters and the hydration of aldehydes
CA-II - Carbonic Anhydrase Type II Isozyme. Looking for abbreviations of CA-II? It is Carbonic Anhydrase Type II Isozyme. Carbonic Anhydrase Type II Isozyme listed as CA-II. Carbonic Anhydrase Type II Isozyme - How is Carbonic Anhydrase Type II Isozyme abbreviated Ett zinkhaltigt erytrocytenzym med molekylvikten 30 kD. Det hör till de mest aktiva av alla kända enzymer och katalyserar den reversibla hydreringen av koldioxid, vilket är av betydelse för transporten av CO2 från kroppsvävnaderna till lungorna. Enzymet blockeras av acetazolamid. EC 18.104.22.168
Carbonic Anhydrase II (CA2) Histone 3 (H3) Tumor Protein P53 (TP53) Epidermal Growth Factor Receptor (EGFR) Mast/stem Cell Growth Factor Receptor (KIT) Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) CD4 Molecule (CD4) tau Protein; Tumor Necrosis Factor alpha (TNF Carbonic anhydrase II: Background: Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Synonyms Carbonic Anhydrase Functions the alpha form is found in humans. It's an enzyme that contains a pocket of amino acids His94, His96, and His119 that hold a zinc ion. when CO2 is introduced into the active site of the enzyme, it gains an OH- that is bonded to the zinc, which then produced the carbonic acid that is subsequently released
ProductName Carbonic Anhydrase II from Human, Recombinant. EC No. EC 22.214.171.124. CAS No. 9001-03-. Source E. coli Inquiry + See More >> Related Protocol Carbonic anhydrase II (CAII)Cre and CreER TM constructs were generated with 1.6-kb human CAII promoter from pCAIIXbaI-TtH111I (a gift from T. Takeya, Nara Institute of Science and Technology, Nara, Japan) as described previously , and Cre or CreER TM coding sequence from pPDXPB CreER TM carbonic anhydrase II. carbonic anhydrase II: translation (CAII) An enzyme present in erythrocytes which catalyses the formation of carbonic acid by water and carbon dioxide, and also the decomposition of carbonic acid. Forensic science glossary. John C. Brenner
Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease Ribbon diagram of human carbonic anhydrase II. Active site zinc ion visible at center Close-up of active site of human carbonic anhydrase II, showing three histidine residues (in pink) and a hydroxide group (red and white) coordinating the zinc ion (purple) Andersson D, Freskgård P-O, Jonsson B-H, Carlsson U (1997) Formation of local native-like tertiary structures in the slow refolding reaction of human carbonic anhydrase II as monitored by CD on tryptophan mutants. Biochemistry 36: 4623-4630 PubMed CrossRef Google Schola Here we report a short-term simple colorimetric microplate method which can be applied for analyses of large numbers of samples. Furthermore in this study we characterized full length coding sequence (cDNA) of M. galloprovincialis carbonic anhydrase II (CAII). The CAII cDNA (with the 5' and 3' untranslated regions) is 1317 bp long The wireframe display in the left screen shows the structure of the bovine carbonic anhydrase II, one of its seven (I to VII) isozymes. It contains 259 amino acid residues with a molecular weight of 29 kDa. The six α-helices (red) and 10 β-sheets (blue) make up the secondary structure of this monomeric enzyme
In normal lung, the predominant cytoplasmic carbonic anhydrase (CA) isozyme (CAII) is highly expressed in amine- and peptide-producing pulmonary neuroendocrine cells where its role involves CO 2 sensing. Here, we report robust cytoplasmic expression of CAII by immunohistochemistry in the tumor cells of different native neuroendocrine tumor (NET) types, including typical and atypical carcinoids. Carbonic anhydrase II (CA II) deficiency is an extremely rare autosomal recessive disorder, characterised by a triad of osteopetrosis, renal tubular acidosis and cerebral calcifications. A 12-year-old boy with classical features of CA II deficienc Carbonic anhydrases are zinc metalloenzymes that catalyse the reversible hydration of carbon dioxide to bicarbonate. Carbonic anhydrase II binds to Na+/H+ exchanger altering pH . Carbonic anhydrases also catalyse the reversible hydration of CO2, HCO 3 - and H+ . Therefore, the role of carbonic anhydrases in maintaining the p
In humans, carbonic anhydrase exists as a number of isoenzymes, the most active being carbonic anhydrase II (CA-II), found primarily in red blood cells (RBCs), but also in other tissues. Inhibition of carbonic anhydrase in the ciliary processes of the eye decreases aqueous humor secretion, presumably by slowing the formation of bicarbonate ions with subsequent reduction in sodium and fluid transport Carbonic Anhydrase Isozyme II human. catalog : C6165. more info or order : MilliporeSigma product webpage. citations: 104. Reference. Tabbì G, Magrì A, Rizzarelli E. The copper (II) binding centres of carbonic anhydrase are differently affected by reductants that ensure the redox intracellular environment Carbonic Anhydrase II Antibody detects Carbonic Anhydrase II. Carbonic Anhydrase II is essential for bone resorption and osteoclast differentiation by similarity. It can hydrate cyanamide to urea and is involved in the regulation of fluid secretion into the anterior chamber of the eye. Anti-Carbonic Anhydrase II Antibody is ideal for investigators involved in Cell Signaling, Neuroscience.
Human carbonic anhydrase isoenzymes I and II (HCA I and II) were purified from human erythrocytes by inhibitor affinity chromatography and ion-exchange chromatography. These isoenzymes were then located in the human adrenal gland using specific polyclonal antisera raised in rabbits and specific detection by immunohistochemical techniques Carbonic Anhydrase II is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings).Descriptors are arranged in a hierarchical structure, which enables searching at various levels of specificity Carbonic anhydrases are zinc metalloenzymes which catalyse the reversible hydration of carbon dioxide to bicarbonate ( PUBMED:18336305 ), ( PUBMED:10978542 ). CAs have essential roles in facilitating the transport of carbon dioxide and protons in the intracellular space, across biological membranes and in the layers of the extracellular space; they.
Another family of key proteins in tumor acid/base regulation is the carbonic anhydrases, which catalyze the reversible hydration of CO 2 to HCO 3- + H +. The role of the cancer-specific isoform CAIX in tumor development and progression has been studied extensively, but physiological data on CAII in cancer cells are still relatively scarce LifeSpan BioSciences currently sells 146 antibodies , 4 peptides , 33 ELISA Kits , 25 proteins specific for CA2 / Carbonic Anhydrase II. Products Research Area In the present study, we used carbonic anhydrase (CA) isozyme II as a model to investigate the effects of acetaldehyde reaction with the protein. CA II is a well characterized enzyme expressed in several organs, including the brain, stomach, gut, kidney, and reproductive organs, and it is highly abundant in erythrocytes [ 40 ] . The pathogenesis of the mental subnormality and cerebral calcification is poorly understood; however, CA II deficiency provides significant insight concerning CA II in renal regulation of acid/base homeostasis and osteoclast-mediated bone resorption
Human carbonic anhydrase II (hCA II) is a zinc metalloenzyme that catalyzes the reversible hydration/dehydration of CO 2 /HCO 3. Although hCAII has been extensively studied to investigate the proton-transfer process that occurs in the active site, its underlying mechanism is still not fully understood. Here The anti-Carbonic Anhydrase II/CA2 antibody (PB9989) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free Carbonic anhydrase (CA) II and IX are enzymes involved in pH homeostasis and have been shown to be upregulated in several types of cancer. In this study, the authors evaluate the expression of CA II and IX in meningiomas and assess their relationship to patient age, tumor type and grade, tumor sex hormone receptor status, tumor cell proliferation, and tumor recurrence Carbonic anhydrase II, Ki: 1.9 nM: S3178: Brinzolamide: Brinzolamide is a potent carbonic anhydrase II inhibitor with IC50 of 3.19 nM. Selective: CAII, IC50: 3.19 nM: S4039: Methazolamide: Methazolamide is a carbonic anhydrase inhibitor with K i of 50 nM, 14 nM and 36 nM for hCA I, hCA II and bCA IV isoforms, respectively. Pan: hCAII, Ki: 14 nM.
Radiographs of 18 children thought to have carbonic anhydrase II deficiency were reviewed to assess intracranial calcification. These children have an autosomal recessive syndrome consisting of osteopetrosis, renal tubular acidosis, intracranial calcification, and developmental retardation . It is widespread and found in archaea, bacteria, and eukaryotes. Three distinct classes exist, and appear to have evolved independently
The carbonic anhydrase II isozyme is found in bone, kidney, and brain, which is why the defects occur in these tissues when the enzyme is deficient or non-functional. The carbonic anhydrase II enzyme is highly active, with one of the highest turnover rates of any known enzyme, and is critical in maintaining proper acid-base balance Carbonic Anhydrase II (CA2) Interferon gamma (IFNG) Histone 3 (H3) Interleukin 10 (IL10) Interleukin 4 (IL4) Interleukin 6 (IL6) Tumor Necrosis Factor alpha (TNF) Interleukin 1, beta (IL1B) Interleukin 2 (IL2) IgG; Interleukin 8 (IL8) Interferon alpha (IFNA) Insulin-Like Growth Factor 1 (IGF1) Interleukin 1 alpha (IL1A) Interleukin 12 (IL12. Carbonic anhydrase III occurred alone in some and with CA II in other sites but was often absent from CA‐II‐containing types of cells. This was exemplified by CA III's abundance in CA‐II‐positive proximal colon and its sparsity in the CA‐II‐rich distal colon of the mouse
Carbonic anhydrase II (CAII) plays an important role in carbon dioxide metabolism and intracellular pH regulation. In this study, we provide evidence that CAII is expressed in both type I (AECI) and type II (AECII) alveolar epithelial cells by RT-PCR and Western blotting in freshly isolated rat cells Carbonic anhydrase IV & II in non-pigmented ciliary epithelial cells. Investigative Ophthalmology and Visual Science . 1996 Feb 15;37(3):S1108. Wu, Qiang ; Delamere, Nicholas A. ; Pierce, William M. / Carbonic anhydrase IV & II in non-pigmented ciliary epithelial cells Carbonic Anhydrase Substrate Product (Absorbance: 405 nm) II. Applications: Detection of CA activity in hemolysates and serum. Determination of enzymatic activity of purified CA. III. Sample Type: Hemolysate, serum Purified CA IV. Kit Contents: Components K472-100 Cap. F. Pacchiano, M. Aggarwal, B. S. Avvaru et al., Selective hydrophobic pocket binding observed within the carbonic anhydrase II active site accommodate different 4-substituted-ureido-benzenesulfonamides and correlate to inhibitor potency, Chemical Communications, vol. 46, no. 44, pp. 8371-8373, 2010. View at: Publisher Site | Google Schola
Objective Carbonic anhydrase (CA) II deficiency is a rare autosomal recessive disorder caused by mutation in the CA II gene that leads to osteopetrosis, renal t.. This Anti-Carbonic Anhydrase II Antibody, bovine erythrocyte is validated for use in ELISA, IP, WB for the detection of Carbonic Anhydrase II. - Find MSDS or SDS, a COA, data sheets and more information In the presence of human carbonic anhydrase II, aryl-sulfonamide-bearing IrCp* pianostool complexes catalyze the asymmetric transfer hydrogenation of imines. Critical cofactor-protein interactions revealed by the X-ray structure of [(η5-Cp*)Ir(pico 4)Cl] 9 ⊂ WT hCA II were genetically optimized to improve t Deficiency of carbonic anhydrase II (carbonate hydro-lyase, EC 126.96.36.199) is the primary defect in the syndrome of osteopetrosis, renal tubular acidosis, and cerebral calcification. In this report we describe the molecular basis for carbonic anhydrase II deficiency in the American family in which the association of carbonic anhydrase II deficiency with this syndrome was first recognized Carbonic anhydrase III is found in high concentration in muscle. It shows relatively poor hydratase and esterase activities compared to the red cell isozymes CA I and CA II (), but is similar in subunit structure (monomer) and molecular mass (28 kD) (Carter et al., 1979).Wade et al. (1986) identified a CA3 mRNA transcript from an adult human muscle cDNA library and presented the complete. Human carbonic anhydrase isozymes CA I and CA II were prepared in our laboratory from erythrocytes provided by the hospital Blood Bank. All other chemicals were reagent grade. 851. 852 Wistrand and Knuuttila: Renal membrane-bound carbonic anhydrase Preparation of renal microsomes and plasma membrane